1) Adam Stewart (d. 20 June 1575), son of Lady Elizabeth Stewart (daughter of John Stewart, 3rd Earl of Lennox.) Prior of Charterhouse, Perth. Buried at St. Magnus, Kirkwall, Orkney; tombstone survives. 2) James Stewart, son of Christine Barclay 3) Jean Stewart (d. 7 January 1588), daughter of Elizabeth Bethune. Married Archibald Campbell, 5th Earl of Argyll in 1553, divorced in 1573 due to desertion. 4) James Stewart (c. 1529–57), son of Elizabeth Shaw. Commendator of Kelso and Melrose. 5) Robert Stewart, 1st Earl of Orkney (b.1533), son of Euphame Elphinstone Prior and Commendator of Holyrood Abbey. 6) John Stewart, Lord Darnley and Prior of Coldingham, (c. 1531 – November 1563), son of Elizabeth Carmichael (1514–1550) who later married John Somerville of Cambusnethan. He married Jean (or Jane) Hepburn, sister and heiress of James Hepburn, 4th Earl of Bothwell, their son Francis Stewart became Earl of Bothwell and a daughter Christine Stewart was appointed to rock the cradle of Prince James in March 1567. 7) James Stewart, 1st Earl of Moray, son of Margaret Erskine, James's favourite mistress. Prior of St Andrews, Advisor and rival to his half-sister, Mary, Queen of Scots and regent for his nephew, James VI. 8) Robert Stewart, junior, (d. 1581), mother unknown. Prior of Whithorn. 9) Margaret Stewart, mother unknown.